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Published
27-12-2024
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Research Articles
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Copyright (c) 2024 Muhammad Awais, Gulshan Irshad, Sumaira Maqsood, Muhammad Inam-ul- Haq, Asim Gulzar, Muhammad Kashif, Ammar Mehmood, Iqra Shoukat, Muhammad Irfan, Muhammad Saim Abbasi
This work is licensed under a Creative Commons Attribution 4.0 International License.
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OPTIMIZATION OF CULTURE MEDIA AND GRAIN SUBSTRATES FOR INDUSTRIAL SPAWN PRODUCTION OF CALOCYBE INDICA
Muhammad Awais
Department of Plant Pathology, PMAS Arid Agriculture University Rawalpindi, Pakistan
Gulshan Irshad
Department of Plant Pathology, PMAS Arid Agriculture University Rawalpindi, Pakistan
Sumaira Maqsood
Department of Environmental Sciences, Kohsar University Murree, Pakistan
Muhammad Inam-ul- Haq
Department of Plant Pathology, PMAS Arid Agriculture University Rawalpindi, Pakistan
Asim Gulzar
Department of Entomology, PMAS Arid Agriculture University Rawalpindi, Pakistan
Muhammad Kashif
Department of Plant Pathology, PMAS Arid Agriculture University Rawalpindi, Pakistan
Ammar Mehmood
Department of Plant Pathology, PMAS-Arid Agriculture University Rawalpindi
Iqra Shoukat
Department of Botany, Kohsar University Murree, Pakistan
Muhammad Irfan
Department of Forestry and Range Management, Kohsar University Murree, Pakistan
Muhammad Saim Abbasi
Department of Forestry and Range Management, Kohsar University Murree, Pakistan
Keywords: Mass multiplication; Spawn; Milky mushroom
Abstract
Mushrooms are large, fleshy, edible fungi that belong to the Ascomycetes or Basidiomycetes classes. Among them, milky mushrooms (Calocybe indica) are the third most commercially cultivated variety in Pakistan, following button and oyster mushrooms. This study aimed to identify the most effective growing medium and grain substrate for the culture and spawn preparation of milky mushrooms. Three media Potato Dextrose Agar (PDA), malt extract agar, and Czapek Dox Agar (CDA) were evaluated for their influence on mycelial growth. The treatments were designated as T1 (PDA), T2 (MEA), and T3 (CDA). PDA showed significantly superior results in promoting cultural growth. Mycelial development was observed on days 4, 7, and 10. Additionally, grain substrates were assessed, revealing that wheat grains supported the maximum mycelial growth, while oat grains showed the least growth on days 7, 14, and 21. These findings suggest that PDA and wheat grains are the most suitable for mass multiplication of C. indica.
